We analyse proteins both in gel and from solution. Solution samples should be detergent free and around 10ul of 10uM will be sufficient protein to do several runs if required. Gels should be stained (coomassie, silver or sypro) and destained. The samples are reduced, alkylated (gel bands only)and digested (trypsin as standard). The resulting peptides are analysed by either MALDI MS & MSMS or by nLC ESI MS& MSMS. The spectra are searches against NCBI, Swissprot or our internal database using Mascot. The mascot server is accessed here, MASCOT SERVER
Intact Protein Mass
We measure the intact mass of proteins by LCMS. 20ul of 10uM sample is required in a detergent free buffer. Samples are bound to a C4 column, washed free of salts, and eluted directly into the MS. The charged ion series is deconvoluted using Waters MaxEnt algorithm.
We are experienced in SWATH and iTRAQ relative quantitation. Please contact us for further information.