S-Trap™ : An easy-to-use spin column method for sample processing(S-Trap-method). Application includes: sample concentration, clean up and digestion with reduced variability. Use S-Trap™ micros for sub-microgram to 50 µg scales. S-Trap™ minis for 50 – 300 µg and S-Trap™ midis for larger scales (300 µg to multiple mg).
In-solution digestion method is usually a method of choice for comparative proteomic studies. Compared to in-solution digestion, in-gel digestion can be more complicated as it usually involves dicing of gel slices, extensive washing/destaining, and peptide extraction after digestion. These multiple steps may increase the variability and render in-gel digestion less reproducible than in-solution digestion. In solution digestionSP3 beads method using sp3 bead (single-pot solid-phase-enhanced sample preparation), is a technique for sample
preparation in a single tube, an approach that aim at minimizing sample losses by simplifying the proteomic sample preparation workflow. Note:Sample preparation steps differs based on the complexity and chemistry of the sample. In this website we provide few protocols, however please discuss with our staff to make the right choice for your sample.
Excised gel bands are cut into small pieces and processed for In gel digestion. In comparative proteomic studies, it is important to know the variability associated with sample preparation. Sample preparation steps differs based on the complexity and chemistry of the sample. In this website we provide few protocols, however please discuss with our staff to make the right choice for your sample.