19 articles found

New Fusion Lumos

Our new mass spectrometer is installed and operational.  With BBSRC 19Alert equipment grant funding we purchased a Thermoscientific Fusion Lumos orbitrap mass spectrometer with a standard HPLC (Vanquish) and nano LC (Ultimate). We look forward to familiarising ourselves with the instrument and getting some nice analysis on it soon

New Mascot server

We have recently upgraded our mascot server hardware.  The new link to the server is https://newbsrcmascot.st-andrews.ac.uk/mascot/ .  The login and password will remain the same as before.

Facility fully open

The BSRC mass spectrometry and proteomics facility is fully open and able to analyse your samples.  We aim to have at least one member of staff in the facility each day, and can always be contacted on massspec @ st-andrews.ac.uk

Temporary suspension on on-line sample submission

We have had a few issues with the on-line sample submission that we set up at the start of the year.  Whilst we sort out these problems we have reverted back to your previous paper submission.  Links to the form to print out can be found in the sample submission page.

In-solution method using S-Trap column

S-Trap™ : An easy-to-use spin column method for sample processing(S-Trap-method). Application includes: sample concentration, clean up and digestion with reduced variability. Use S-Trap™ micros for sub-microgram to 50 µg scales. S-Trap™ minis for 50 – 300 µg and S-Trap™ midis for larger scales (300 µg to multiple mg).

In-solution digestion SP3 beads

In-solution digestion method is usually a method of choice for comparative proteomic studies. Compared to in-solution digestion, in-gel digestion can be more complicated as it usually involves dicing of gel slices, extensive washing/destaining, and peptide extraction after digestion. These multiple steps may increase the variability and render in-gel digestion less reproducible than in-solution digestion. In…

In-gel digestion of proteins

Excised gel bands are cut into small pieces and processed for In gel digestion. In comparative proteomic studies, it is important to know the variability associated with sample preparation. Sample preparation steps differs based on the complexity and chemistry of the sample.  In this website we provide few protocols, however please discuss with our staff…

Temporary suspension of Intact Mass Analysis

On the 17th April we will retiring the LCT mass spectrometer after many years of faithful service.  This is the instrument that we use to measure the intact mass of proteins.  However on the 29th April we are getting a new Xevo G2 Tof to carry out this analysis.  It will take a week to…

Biomolecular Sciences Building Fire

Here is a picture of the fire, we are so lucky that we are unscathed, but our thoughts are with the many research groups who are affected and have lost so much research and equipment The Mass Spectrometry and Proteomics Facility is unaffected by the fire in the main BMS building.  The facility is in…